dc.contributor.author | Kaba, S.A. | en_US |
dc.contributor.author | Nene, Vishvanath | en_US |
dc.contributor.author | Musoke, A.J. | en_US |
dc.contributor.author | Vlak, J.M. | en_US |
dc.contributor.author | Oers, M.M. van | en_US |
dc.date.accessioned | 2023-12-29T09:46:44Z | en_US |
dc.date.available | 2023-12-29T09:46:44Z | en_US |
dc.identifier.uri | https://hdl.handle.net/10568/136002 | en_US |
dc.title | Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells | en_US |
cg.authorship.types | CGIAR and advanced research institute | en_US |
dcterms.abstract | East Coast fever (ECF) is a fatal disease of cattle caused by the protozoan parasite Theileria parva. The development of a subunit vaccine, based on the sporozoite-specific surface antigen p67, has been hampered by difficulties in achieving high-level expression of recombinant p67 in a near-authentic form. Therefore two sets of recombinant baculovirus vectors were constructed. The first set, encoding various regions of p67, produced low levels of the corresponding p67 domains in High Five™ cells, despite the presence of large amounts of p67 RNA. The second, consisting of p67 domains fused to the carboxy-terminus of GFP expressed significantly higher levels of p67 protein. The GFP[ratio ]p67 fusion proteins were recognized by a sporozoite-neutralizing monoclonal antibody (TpM12) raised against native p67 whereas non-fused full length p67 expressed in insect cells was not recognized. GFP-tagging therefore, appeared to enhance the stability of p67 and to conserve its folding. The high-level expression of p67 domains in a more authentic form is an important step towards the development of an effective subunit vaccine against ECF. | en_US |
dcterms.accessRights | Limited Access | en_US |
dcterms.audience | Scientists | en_US |
dcterms.bibliographicCitation | Kaba, S.A., Nene, V., Musoke, A.J., Vlak, J.M. and Oers, M.M. Van. 2002. Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells. Parasitology 125(6): 497–505. | en_US |
dcterms.issued | 2002-06 | en_US |
dcterms.language | en | en_US |
dcterms.license | Other | en_US |
dcterms.subject | animal diseases | en_US |
dcterms.subject | east coast fever | en_US |
dcterms.type | Journal Article | en_US |
cg.subject.ilri | ANIMAL DISEASES | en_US |
cg.subject.ilri | ECF | en_US |
cg.contributor.affiliation | Wageningen University | en_US |
cg.contributor.affiliation | International Livestock Research Institute | en_US |
cg.identifier.doi | https://doi.org/10.1017/S003118200200241X | en_US |
cg.isijournal | ISI Journal | en_US |
cg.creator.identifier | Vishvanath Nene: 0000-0001-7066-4169 | en_US |
cg.reviewStatus | Peer Review | en_US |
cg.howPublished | Formally Published | en_US |
cg.journal | Parasitology | en_US |
cg.issn | 1469-8161 | en_US |